andrographolide estimation method

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Spectrophotometric determination of andrographolides in Andrographis paniculata nees and its formulation

13/3/2005 · The method is based on the condensation of g-unsaturated lactone ring of andrographolides and picric acid in alkaline medium which results in the formation of colored complex, that which could be measured at 494 nm. Linearity of the proposed

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DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR ESTIMATION OF HEPATOPROTECTIVE DITERPENOID ANDROGRAPHOLIDE IN POLYHERBAL FORMULATIONS

The precision of the method in terms of intra-day precision (% RSD) was determined by analysing andrographolide standard solutions in the range (200-1000 ng/spot) three times on the same day. Inter-day precision (% RSD) was assessed by analysing

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(PDF) Spectrophotometric determination of andrographolides in Andrographis paniculata nees and its formulation

A simple, sensitive and reproducible spectrophotometric method was developed for the estimation of andrographolides in Andrographis paniculata (Kalmegh) and its formulation. The method is based on…

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Quantitative Determination of Andrographolide and Related Compounds in Andrographis paniculata Extracts and Biological Evaluation of Their Anti

The method was proved to be suitable for the quantitation of these three diterpenes in four different commercial Andrographis dietary supplements. The anti-inflammatory activities of a mixture of known composition have been evaluated showing

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Simultaneous estimation of andrographolide and wedelolactone in herbal formulations.

The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 400 ng/spot for andrographolide and 100 to 200 ng/spot for wedelolactone.

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Andrographolide: A Review of Analytical Methods | Journal of Chromatographic Science | Oxford Academic

This method was employed for the determination of andrographolide which not only minimizes the use of organic solvents but also combines the extraction and preconcentration in a single step. The non-ionic surfactant Triton X-114 was chosen for

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High-performance thin layer chromatography method for estimation of andrographolide in herbal extract and polyherbal formulations

ORIGINAL ARTICLE High-performance thin layer chromatography method for estimation of andrographolide in herbal extract and polyherbal formulations Punit K. Jain a, V. Ravichandran a,b, Prateek K. Jain a, Ram K. Agrawal a,* a Pharmaceutical

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Simultaneous Estimation of Andrographolide and Wedelolactone in Herbal Formulations

analytical method for the simultaneous estimation of the andrographolide and wedelolactone as markers of the individual drugs in herbal formulations. All the solvents and reagents of analytical grade purchased from M/s.Qualigens India Ltd. were

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(PDF) Validated HPLC Method for Determination of Andrographolide in Mixed Herbal Extract

The objective of this research was development and validation of RP-HPLC method for quantification of andrographolide in herbal extracts containing extract of Andrographis paniculata (Burm f.)…

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Spectrophotometric determination of total lactones in Andrographis paniculata Nees

The reaction between the Kedde’s reagentsand andrographolide is fast and rapid compared tothe titration method described in the Thai Phar-macopoeia. The HPLC method requires a moresophisticated instrument, pure components asstandards. This metho

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Estimation of Adrographolide in Andrographis paniculata Herb, Extracts and Dosage forms

Various methods have been suggested for quantitative estimation of andrographolide in Andrographis paniculata. The gravimetric method described in the Indian pharmaco-poeia was found to give high values [16]. This is due to some yellow coloring

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High-performance thin layer chromatography method for estimation of andrographolide in herbal extract and polyherbal formulations – ScienceDirect

1/10/2010 · A new, simple, sensitive, precise and robust high-performance thin layer chromatographic (HPTLC) method was developed for the estimation of andrographolide in herbal extracts and pharmaceutical dosage forms. Analysis of andrographolide was perfo

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DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR THE DETERMINATION OF ANDROGRAPHOLIDE IN KALMEGH NAVAYAS LOHA AN AYURVEDIC FORMULATION

Research Article DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR THE DETERMINATION OF ANDROGRAPHOLIDE IN KALMEGH NAVAYAS LOHA AN AYURVEDIC FORMULATION PAWAR 1R.K. 2, SHARMA SHIVANI , SINGH K.C. 2 AND SHARMA RAJEEV KR.

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High-performance thin layer chromatography method for estimation of andrographolide in herbal extract and polyherbal formulations. – CAB Direct

A new, simple, sensitive, precise and robust high-performance thin layer chromatography (HPTLC) was developed for the estimation of andrographolide from Andrographis paniculata in herbal extracts and pharmaceutical dosage forms. Analysis of andr

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A Validated Method for the Quality Control of Andrographis paniculata Preparations

The overall method was validated for precision and accuracy over at least three different concentration levels. Relative standard deviation was less than 1.13%, whereas recovery was between 95.50% and 97.19%. The method also proved to be

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